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and a control empty vector (con136)  (Genechem)

 
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    Genechem and a control empty vector (con136)
    Analyses of SIRT1 subcellular location and expression in ovarian carcinoma IGROV1 cells overexpressing SIRT1 (SIRT1 cells) or NLS-mutated SIRT1 (SIRT1 NLSmt cells). a The NLS sequence located at amino acids 33–40 (LRKRP RR D) (CTCCGCAAGAGGCCG CGGAGA GAT) was mutated to LRKRP AA D (CTCCGCAAGAGGCCG GCTGCC GAT). In addition, the other NLS sequence located at amino acids 231–238 (PPKR KKRK ) (CCACCAAAAAGG AAAAAAAGAAAA ) was mutated to PPKR AAAA (CCACCAAAAAGG GCCGCTGCTGCC ). b SIRT1 mRNA levels as measured by real-time PCR in the parental (IGROV1), <t>Con136,</t> SIRT1 and SIRT1 NLSmt cells. Each bar represents the mean ± SD ( n = 3; ** P < 0.01; ns , not significant). c Western blot analyses of SIRT1 expression in Con136, SIRT1 and SIRT1 NLSmt cells. β-Actin was used as a loading control. d Immunofluorescence staining of GFP in parental (IGROV1), Con136, SIRT1 and SIRT1 NLSmt cells (original magnification: × 400; scale bars: 20 μm). Boxes show the cell details at a higher magnification (scale bars: 20 μm). e SIRT1 expression in cytoplasmic and nuclear extracts from Con136, SIRT1 and SIRT1 NLSmt cells. β-tubulin and lamin B were used as cytoplasmic and nuclear loading controls, respectively. f Protein levels of SIRT1, p53 and acetylated (K382) p53 before and after treatment with 1 μg/ml for 24 h in Con136, SIRT1 and SIRT1 NLSmt cells as analyzed by western blotting. β-Actin was used as a loading control
    And A Control Empty Vector (Con136), supplied by Genechem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/and a control empty vector (con136)/product/Genechem
    Average 90 stars, based on 1 article reviews
    and a control empty vector (con136) - by Bioz Stars, 2026-03
    90/100 stars

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    1) Product Images from "Cytoplasmic SIRT1 inhibits cell migration and invasion by impeding epithelial–mesenchymal transition in ovarian carcinoma"

    Article Title: Cytoplasmic SIRT1 inhibits cell migration and invasion by impeding epithelial–mesenchymal transition in ovarian carcinoma

    Journal: Molecular and Cellular Biochemistry

    doi: 10.1007/s11010-019-03559-y

    Analyses of SIRT1 subcellular location and expression in ovarian carcinoma IGROV1 cells overexpressing SIRT1 (SIRT1 cells) or NLS-mutated SIRT1 (SIRT1 NLSmt cells). a The NLS sequence located at amino acids 33–40 (LRKRP RR D) (CTCCGCAAGAGGCCG CGGAGA GAT) was mutated to LRKRP AA D (CTCCGCAAGAGGCCG GCTGCC GAT). In addition, the other NLS sequence located at amino acids 231–238 (PPKR KKRK ) (CCACCAAAAAGG AAAAAAAGAAAA ) was mutated to PPKR AAAA (CCACCAAAAAGG GCCGCTGCTGCC ). b SIRT1 mRNA levels as measured by real-time PCR in the parental (IGROV1), Con136, SIRT1 and SIRT1 NLSmt cells. Each bar represents the mean ± SD ( n = 3; ** P < 0.01; ns , not significant). c Western blot analyses of SIRT1 expression in Con136, SIRT1 and SIRT1 NLSmt cells. β-Actin was used as a loading control. d Immunofluorescence staining of GFP in parental (IGROV1), Con136, SIRT1 and SIRT1 NLSmt cells (original magnification: × 400; scale bars: 20 μm). Boxes show the cell details at a higher magnification (scale bars: 20 μm). e SIRT1 expression in cytoplasmic and nuclear extracts from Con136, SIRT1 and SIRT1 NLSmt cells. β-tubulin and lamin B were used as cytoplasmic and nuclear loading controls, respectively. f Protein levels of SIRT1, p53 and acetylated (K382) p53 before and after treatment with 1 μg/ml for 24 h in Con136, SIRT1 and SIRT1 NLSmt cells as analyzed by western blotting. β-Actin was used as a loading control
    Figure Legend Snippet: Analyses of SIRT1 subcellular location and expression in ovarian carcinoma IGROV1 cells overexpressing SIRT1 (SIRT1 cells) or NLS-mutated SIRT1 (SIRT1 NLSmt cells). a The NLS sequence located at amino acids 33–40 (LRKRP RR D) (CTCCGCAAGAGGCCG CGGAGA GAT) was mutated to LRKRP AA D (CTCCGCAAGAGGCCG GCTGCC GAT). In addition, the other NLS sequence located at amino acids 231–238 (PPKR KKRK ) (CCACCAAAAAGG AAAAAAAGAAAA ) was mutated to PPKR AAAA (CCACCAAAAAGG GCCGCTGCTGCC ). b SIRT1 mRNA levels as measured by real-time PCR in the parental (IGROV1), Con136, SIRT1 and SIRT1 NLSmt cells. Each bar represents the mean ± SD ( n = 3; ** P < 0.01; ns , not significant). c Western blot analyses of SIRT1 expression in Con136, SIRT1 and SIRT1 NLSmt cells. β-Actin was used as a loading control. d Immunofluorescence staining of GFP in parental (IGROV1), Con136, SIRT1 and SIRT1 NLSmt cells (original magnification: × 400; scale bars: 20 μm). Boxes show the cell details at a higher magnification (scale bars: 20 μm). e SIRT1 expression in cytoplasmic and nuclear extracts from Con136, SIRT1 and SIRT1 NLSmt cells. β-tubulin and lamin B were used as cytoplasmic and nuclear loading controls, respectively. f Protein levels of SIRT1, p53 and acetylated (K382) p53 before and after treatment with 1 μg/ml for 24 h in Con136, SIRT1 and SIRT1 NLSmt cells as analyzed by western blotting. β-Actin was used as a loading control

    Techniques Used: Expressing, Sequencing, Real-time Polymerase Chain Reaction, Western Blot, Immunofluorescence, Staining

    Overexpression of SIRT1 and SIRT1 NLSmt had opposite effects on cell migration and invasion. Con136, SIRT1, and SIRT1 NLSmt cells passing through the chamber were methanol-fixed, crystal violet-stained, and counted in a × 200 microscopic field. Representative photomicrographs of the cell migration ( a ) and invasion ( b ) assays are shown (scale bars: 50 μm). Relative quantification of migration ( c ) and invasion ( d ) was performed by normalization to the migration and invasion of Con136 cells. The error bars represent the means ± SDs ( n = 10; *** P < 0.001)
    Figure Legend Snippet: Overexpression of SIRT1 and SIRT1 NLSmt had opposite effects on cell migration and invasion. Con136, SIRT1, and SIRT1 NLSmt cells passing through the chamber were methanol-fixed, crystal violet-stained, and counted in a × 200 microscopic field. Representative photomicrographs of the cell migration ( a ) and invasion ( b ) assays are shown (scale bars: 50 μm). Relative quantification of migration ( c ) and invasion ( d ) was performed by normalization to the migration and invasion of Con136 cells. The error bars represent the means ± SDs ( n = 10; *** P < 0.001)

    Techniques Used: Over Expression, Migration, Staining

    Comparison of the expression levels of EMT-associated proteins in the Con136, SIRT1, and SIRT1 NLSmt cells. Relative mRNA levels of vimentin ( a ), fibronectin ( b ), CK-18 ( c ), CK-19 ( d ), plakophilin-2 ( e ), plakophilin-3 ( f ), epiplakin ( g ), and nectin-1 ( h ) as measured by real-time PCR in the Con136, SIRT1, and SIRT1 NLSmt cells. Each bar represents the mean ± SD ( n = 3; *** P < 0.001; ** P < 0.01; * P < 0.05; ns not significant). i Protein levels of vimentin, CK-18 and CK-19 as determined by western blot analyses of the Con136, SIRT1, and SIRT1 NLSmt cells. β-Actin was used as a loading control
    Figure Legend Snippet: Comparison of the expression levels of EMT-associated proteins in the Con136, SIRT1, and SIRT1 NLSmt cells. Relative mRNA levels of vimentin ( a ), fibronectin ( b ), CK-18 ( c ), CK-19 ( d ), plakophilin-2 ( e ), plakophilin-3 ( f ), epiplakin ( g ), and nectin-1 ( h ) as measured by real-time PCR in the Con136, SIRT1, and SIRT1 NLSmt cells. Each bar represents the mean ± SD ( n = 3; *** P < 0.001; ** P < 0.01; * P < 0.05; ns not significant). i Protein levels of vimentin, CK-18 and CK-19 as determined by western blot analyses of the Con136, SIRT1, and SIRT1 NLSmt cells. β-Actin was used as a loading control

    Techniques Used: Expressing, Real-time Polymerase Chain Reaction, Western Blot



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    and a control empty vector (con136)  (Genechem)
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    Genechem and a control empty vector (con136)
    Analyses of SIRT1 subcellular location and expression in ovarian carcinoma IGROV1 cells overexpressing SIRT1 (SIRT1 cells) or NLS-mutated SIRT1 (SIRT1 NLSmt cells). a The NLS sequence located at amino acids 33–40 (LRKRP RR D) (CTCCGCAAGAGGCCG CGGAGA GAT) was mutated to LRKRP AA D (CTCCGCAAGAGGCCG GCTGCC GAT). In addition, the other NLS sequence located at amino acids 231–238 (PPKR KKRK ) (CCACCAAAAAGG AAAAAAAGAAAA ) was mutated to PPKR AAAA (CCACCAAAAAGG GCCGCTGCTGCC ). b SIRT1 mRNA levels as measured by real-time PCR in the parental (IGROV1), <t>Con136,</t> SIRT1 and SIRT1 NLSmt cells. Each bar represents the mean ± SD ( n = 3; ** P < 0.01; ns , not significant). c Western blot analyses of SIRT1 expression in Con136, SIRT1 and SIRT1 NLSmt cells. β-Actin was used as a loading control. d Immunofluorescence staining of GFP in parental (IGROV1), Con136, SIRT1 and SIRT1 NLSmt cells (original magnification: × 400; scale bars: 20 μm). Boxes show the cell details at a higher magnification (scale bars: 20 μm). e SIRT1 expression in cytoplasmic and nuclear extracts from Con136, SIRT1 and SIRT1 NLSmt cells. β-tubulin and lamin B were used as cytoplasmic and nuclear loading controls, respectively. f Protein levels of SIRT1, p53 and acetylated (K382) p53 before and after treatment with 1 μg/ml for 24 h in Con136, SIRT1 and SIRT1 NLSmt cells as analyzed by western blotting. β-Actin was used as a loading control
    And A Control Empty Vector (Con136), supplied by Genechem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/and a control empty vector (con136)/product/Genechem
    Average 90 stars, based on 1 article reviews
    and a control empty vector (con136) - by Bioz Stars, 2026-03
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Analyses of SIRT1 subcellular location and expression in ovarian carcinoma IGROV1 cells overexpressing SIRT1 (SIRT1 cells) or NLS-mutated SIRT1 (SIRT1 NLSmt cells). a The NLS sequence located at amino acids 33–40 (LRKRP RR D) (CTCCGCAAGAGGCCG CGGAGA GAT) was mutated to LRKRP AA D (CTCCGCAAGAGGCCG GCTGCC GAT). In addition, the other NLS sequence located at amino acids 231–238 (PPKR KKRK ) (CCACCAAAAAGG AAAAAAAGAAAA ) was mutated to PPKR AAAA (CCACCAAAAAGG GCCGCTGCTGCC ). b SIRT1 mRNA levels as measured by real-time PCR in the parental (IGROV1), Con136, SIRT1 and SIRT1 NLSmt cells. Each bar represents the mean ± SD ( n = 3; ** P < 0.01; ns , not significant). c Western blot analyses of SIRT1 expression in Con136, SIRT1 and SIRT1 NLSmt cells. β-Actin was used as a loading control. d Immunofluorescence staining of GFP in parental (IGROV1), Con136, SIRT1 and SIRT1 NLSmt cells (original magnification: × 400; scale bars: 20 μm). Boxes show the cell details at a higher magnification (scale bars: 20 μm). e SIRT1 expression in cytoplasmic and nuclear extracts from Con136, SIRT1 and SIRT1 NLSmt cells. β-tubulin and lamin B were used as cytoplasmic and nuclear loading controls, respectively. f Protein levels of SIRT1, p53 and acetylated (K382) p53 before and after treatment with 1 μg/ml for 24 h in Con136, SIRT1 and SIRT1 NLSmt cells as analyzed by western blotting. β-Actin was used as a loading control

    Journal: Molecular and Cellular Biochemistry

    Article Title: Cytoplasmic SIRT1 inhibits cell migration and invasion by impeding epithelial–mesenchymal transition in ovarian carcinoma

    doi: 10.1007/s11010-019-03559-y

    Figure Lengend Snippet: Analyses of SIRT1 subcellular location and expression in ovarian carcinoma IGROV1 cells overexpressing SIRT1 (SIRT1 cells) or NLS-mutated SIRT1 (SIRT1 NLSmt cells). a The NLS sequence located at amino acids 33–40 (LRKRP RR D) (CTCCGCAAGAGGCCG CGGAGA GAT) was mutated to LRKRP AA D (CTCCGCAAGAGGCCG GCTGCC GAT). In addition, the other NLS sequence located at amino acids 231–238 (PPKR KKRK ) (CCACCAAAAAGG AAAAAAAGAAAA ) was mutated to PPKR AAAA (CCACCAAAAAGG GCCGCTGCTGCC ). b SIRT1 mRNA levels as measured by real-time PCR in the parental (IGROV1), Con136, SIRT1 and SIRT1 NLSmt cells. Each bar represents the mean ± SD ( n = 3; ** P < 0.01; ns , not significant). c Western blot analyses of SIRT1 expression in Con136, SIRT1 and SIRT1 NLSmt cells. β-Actin was used as a loading control. d Immunofluorescence staining of GFP in parental (IGROV1), Con136, SIRT1 and SIRT1 NLSmt cells (original magnification: × 400; scale bars: 20 μm). Boxes show the cell details at a higher magnification (scale bars: 20 μm). e SIRT1 expression in cytoplasmic and nuclear extracts from Con136, SIRT1 and SIRT1 NLSmt cells. β-tubulin and lamin B were used as cytoplasmic and nuclear loading controls, respectively. f Protein levels of SIRT1, p53 and acetylated (K382) p53 before and after treatment with 1 μg/ml for 24 h in Con136, SIRT1 and SIRT1 NLSmt cells as analyzed by western blotting. β-Actin was used as a loading control

    Article Snippet: Lentiviral vectors with the complete coding sequence of SIRT1 (lenti-SIRT1) and NLS-mutant SIRT1 (lenti-SIRT1 NLSmt ) and a control empty vector (Con136) were purchased from GeneChem (GeneChem, Shanghai, China).

    Techniques: Expressing, Sequencing, Real-time Polymerase Chain Reaction, Western Blot, Immunofluorescence, Staining

    Overexpression of SIRT1 and SIRT1 NLSmt had opposite effects on cell migration and invasion. Con136, SIRT1, and SIRT1 NLSmt cells passing through the chamber were methanol-fixed, crystal violet-stained, and counted in a × 200 microscopic field. Representative photomicrographs of the cell migration ( a ) and invasion ( b ) assays are shown (scale bars: 50 μm). Relative quantification of migration ( c ) and invasion ( d ) was performed by normalization to the migration and invasion of Con136 cells. The error bars represent the means ± SDs ( n = 10; *** P < 0.001)

    Journal: Molecular and Cellular Biochemistry

    Article Title: Cytoplasmic SIRT1 inhibits cell migration and invasion by impeding epithelial–mesenchymal transition in ovarian carcinoma

    doi: 10.1007/s11010-019-03559-y

    Figure Lengend Snippet: Overexpression of SIRT1 and SIRT1 NLSmt had opposite effects on cell migration and invasion. Con136, SIRT1, and SIRT1 NLSmt cells passing through the chamber were methanol-fixed, crystal violet-stained, and counted in a × 200 microscopic field. Representative photomicrographs of the cell migration ( a ) and invasion ( b ) assays are shown (scale bars: 50 μm). Relative quantification of migration ( c ) and invasion ( d ) was performed by normalization to the migration and invasion of Con136 cells. The error bars represent the means ± SDs ( n = 10; *** P < 0.001)

    Article Snippet: Lentiviral vectors with the complete coding sequence of SIRT1 (lenti-SIRT1) and NLS-mutant SIRT1 (lenti-SIRT1 NLSmt ) and a control empty vector (Con136) were purchased from GeneChem (GeneChem, Shanghai, China).

    Techniques: Over Expression, Migration, Staining

    Comparison of the expression levels of EMT-associated proteins in the Con136, SIRT1, and SIRT1 NLSmt cells. Relative mRNA levels of vimentin ( a ), fibronectin ( b ), CK-18 ( c ), CK-19 ( d ), plakophilin-2 ( e ), plakophilin-3 ( f ), epiplakin ( g ), and nectin-1 ( h ) as measured by real-time PCR in the Con136, SIRT1, and SIRT1 NLSmt cells. Each bar represents the mean ± SD ( n = 3; *** P < 0.001; ** P < 0.01; * P < 0.05; ns not significant). i Protein levels of vimentin, CK-18 and CK-19 as determined by western blot analyses of the Con136, SIRT1, and SIRT1 NLSmt cells. β-Actin was used as a loading control

    Journal: Molecular and Cellular Biochemistry

    Article Title: Cytoplasmic SIRT1 inhibits cell migration and invasion by impeding epithelial–mesenchymal transition in ovarian carcinoma

    doi: 10.1007/s11010-019-03559-y

    Figure Lengend Snippet: Comparison of the expression levels of EMT-associated proteins in the Con136, SIRT1, and SIRT1 NLSmt cells. Relative mRNA levels of vimentin ( a ), fibronectin ( b ), CK-18 ( c ), CK-19 ( d ), plakophilin-2 ( e ), plakophilin-3 ( f ), epiplakin ( g ), and nectin-1 ( h ) as measured by real-time PCR in the Con136, SIRT1, and SIRT1 NLSmt cells. Each bar represents the mean ± SD ( n = 3; *** P < 0.001; ** P < 0.01; * P < 0.05; ns not significant). i Protein levels of vimentin, CK-18 and CK-19 as determined by western blot analyses of the Con136, SIRT1, and SIRT1 NLSmt cells. β-Actin was used as a loading control

    Article Snippet: Lentiviral vectors with the complete coding sequence of SIRT1 (lenti-SIRT1) and NLS-mutant SIRT1 (lenti-SIRT1 NLSmt ) and a control empty vector (Con136) were purchased from GeneChem (GeneChem, Shanghai, China).

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Western Blot